The Antarctic toothfish, Dissostichus mawsoni, is a species of Family Nototheniidae of Order Perciformes native to the Southern Ocean and one of the top predators in the Antarctic ocean. We were able to obtain 72 haplotypes (10 orders, 16 Families, 26 Genera, and 35 species) as the five-year metabarcoding analyses about the stomach contents of D. mawsoni in subarea 88.3. Based on the proportions of fish reads (99.61 %) compared with those of the cephalopods (0.27 %), D. mawsoni was piscivorous, which is consistent to the previous studies. Chinobathyscus dewitti (59.09 %), Macrourus caml (20.91%), Magnisudis prionosa (10.69 %), and Lepidonotothen squamifrons (4.52 %) turned out to be the four main preys of D. mawsoni in subarea 88.3. The metabarcoding results in subarea 88.3 were clustered together distinctly from other subarea such as 88.1 and 58.4.1. Those results strongly indicated that metabarcoding analysis of stomach contents of D. mawsoni can reflect the fish assemblage of the specimens collected. Besides regional clustering of stomach contents indicated that prey species of D. mawsoni are regionally different. Finally, the positive correlations between IRI and the proportions of NGS reads were identified with the different R values, which suggested the proportions by the metabarcoding can reflect the biomass of each prey species, albeit with a large degree of variations. For the more accurate estimation of each prey’s biomass, qPCR will be the better method, which would be further used for the long-term surveys with large research areas.

Antarctic toothfish (Dissostichus mawsoni) have been found in regions sampled throughout Subarea 88.3 at depths from 550–2000 m, with size distributions and catch rate (i.e. abundance), differing by area, and especially by depth. Size distributions along the slope were typically bimodal, comprised of both very small and adult fish. Toothfish catch rates were highest on the central portion of the slope (88.3 B). Consistent with observations from other areas, growth of D. mawsoni differed between sexes, with females being larger on average for a given length than males. Resulting von Bertalanffy growth curves were similar to those derived for Antarctic toothfish in the Ross Sea region. Based on histology, length at 50% maturity of female and male Antarctic toothfish was estimated to be 124.5 cm and 124.0 cm, respectively, with length at 95% maturity estimated to be 145.2 cm and 166.3 cm for females and males, respectively. Age at 50% maturity was estimated to be 13.06 years for females and 13.27 years for males. Age at 95% maturity was estimated 18.64 years for females and 21.16 years for males, however greater sampling is required to confirm these parameters. Further sample collection and examination of spatial patterns in the biology and stock structure of D. mawsoni and key bycatch species in Subarea 88.3 are recommended.

In 2019, EU provided a further grant of €100 000 to support CCAMLR to identify and deter illegal, unreported and unregulated (IUU) fishing activities that undermine the objectives of the CAMLR Convention. In order to meet the objectives of the grant, INTERPOL was contracted, and the 2020–2021 activities of the Global Fisheries Enforcement Project are supported. This paper presents the report submitted by INTERPOL in relation to activities between 1 January 2020 to 31 August 2020, including future proposed activities.

We present an update of ageing and estimates of biological parameters, and of the single-region integrated stock assessment for Antarctic toothfish (Dissostichus mawsoni) fishery in CCAMLR Divisions 58.4.1 and 58.4.2. The update of ageing and estimates of biological parameters addresses Milestone 1.4, the updated of the stock assessment addresses Milestone 1.6 of the current multi-member research plan for these Divisions (SC-CAMLR-39/BG/10).

The assessment model used all data available from the region, supplemented with parameter estimates from other toothfish stock assessments. All evaluated assessment models indicated that the Antarctic toothfish stock in Divisions 58.4.1 and 58.4.2 is unlikely to be depleted by the current level of fishing mortality. Accounting for vessel tagging performance was highly influential in the estimates of B₀ and SSB status, and we recommend further work to consolidate appropriate estimates of vessel-specific tag survival and detection performance.

The model indicated unresolved issues with the tagging data and a systematic lack of fit to the catch-at-age data. The lack of directed fishing in Division 58.4.1 resulted in spatially-restricted data collected from a single research block in Division 58.4.2 over the last two fishing season. The models indicated that fishing gear has only a minor influence on catch-at-length and catch-at-age compositions and tag-recapture data in this Antarctic toothfish fishery, particularly relative to vessel and spatial population effects.

Estimates of preliminary catch limits for the two Divisions indicated that the catch limits estimated by the trend analysis are precautionary.

The collaborative approach adopted by the research plan proponents Australia, France, Japan, the Republic of Korea and Spain in Divisions 58.4.1 and 58.4.2 has worked well, with valuable on-water data collection and extensive subsequent data analyses. Based on these analyses, research has now progressed to a stock assessment, highlighting the value of the management procedures, agreed to by CCAMLR in 2011, which requires research plans in exploratory fisheries. However, an expansion of the spatial distribution of catch, tagging and data collection in Divisions 58.4.1 and 58.4.2 beyond a single research block will be required to improve estimates of stock biomass and catch limits in the future.

Robust stock assessments and catch limits for Dissostichus mawsoni according to CCAMLR decision rules remain to be determined for Divisions 58.4.1 and 58.4.2. Precautionary management arrangements are in place as set out in Conservation Measures 41-11 and 41-05.

WG-FSA-16/29, and WG-FSA-18/59 and WG-FSA-19/44 outlined the multi-member toothfish exploratory fishery research plans for these Divisions, including the research objectives, methods, and milestones in accordance with ANNEX 24-01/A.

In this paper, we report on exploratory fishing activities undertaken by Australia, France, Japan, the Republic of Korea, and Spain between the 2011/12 and 2019/20 fishing seasons, including the quantity of data and samples collected, addressing Milestone 1.1 of WG-FSA-19/44.

The summaries provided herein were generated using an R-Markdown document that is available to Members to facilitate standardised reporting on data collection in CCAMLR’s Exploratory Fisheries.

WG-FSA-19 recommended an update on stock parameters, including recruitment indices from the trawl survey, and age-frequency data and tag-recapture data from the fishery be presented to WG-FSA-20 to evaluate whether recruitment and the stock trajectory were consistent with those estimated by the stock assessments for Patagonian toothfish (Dissostichus eleginoides) fishery in CCAMLR Division 58.5.2.

Here, we present an update on stock parameters, including recruitment indices from the trawl survey, and age-frequency data and tag-recapture data from the fishery. These data indicate that the stock trajectory is consistent with that predicted by the 2019 stock assessment model. Increases in the survey biomass and young fish in the survey catch composition also indicate the potential for a recruitment pulse in recent years.

During late March to mid-April 2020, the annual random stratified trawl survey (RSTS) around Heard Island and McDonald Islands (HIMI) was conducted in CCAMLR Division 58.5.2, with the completion of 151 stations. The survey was conducted on the FV Atlas Cove. Sampling protocols such as the design and the duration of the hauls were similar to recent surveys, but with a new set of randomly selected station points. However, only 18 of the 30 stations allocated in Plateau Deep East could be sampled due to damage to the trawl warps which prevented fishing on deeper stations.

The catch of Patagonian toothfish (Dissostichus eleginoides) () was 86.3 t which is the highest catch since the RSTS began and more than three times the average since 2015.  The catch of mackerel icefish (Champsocephalus gunnari) was 7.3 t which is about average.

The estimated biomass of the target species D. eleginoides and C. gunnari in the 2020 survey were the highest for the past 10 years. Biomass estimates for the managed by-catch species unicorn icefish (Champsocephalus rhinoceratus) and Macrourus spp. were also at their highest levels of the past 10 years and the estimate for grey rockcod (Lepidonotothen squamifrons) showed the first substantial increase since 2014. Amongst the three species of skate, biomass estimates show an upward trend over the last few years with of Bathyraja murrayi also being at the highest levels for the past 10 years.

Length and weight measurements were taken for more than 17,000 fish and for more than half of those, other biological measurements were also recorded. Otoliths were collected from 763 D. eleginoides and a number of other species, and 645 toothfish were tagged and released.

The research plan on Antarctic toothfish in Subarea 88.3 has been underway for four consecutive years. Two vessels have participated in tagging and releasing toothfish from 8 discrete areas. Subsequent fishing effort has recaptured some fish, which can now be used to transition the research to a “biomass estimation” phase based on catch limits for each research block following CCAMLR’s trend analysis rules. A total of 2309 tagged fish have been released and 11 recaptured, all in research block 883_4. As proposed in the research plan milestones, we applied the observed recapture rates to the projected catch limits to set expectations of future recaptures using the trend analysis rules to evaluate the likelihood of achieving enough recaptures for stock assessment purposes. We estimate that, at least for research block 883_4, obtaining recaptures of more than 6 tags per year is feasible at the current tagging rate of 5 fish per ton of catch, but only if vessel performance is maintained and within-season recaptures do not occur.

We review the stock structure of Antarctic toothfish Dissostichus mawsoni in Statistical Area 88 with the aim of characterising the likely stock boundaries relative to fishery management. We include information from studies examining genetics, otolith microchemistry, stable isotopes, tagging, size and age structures, growth dynamics, and particle dispersal simulations. A clear progression in the size and age of Antarctic toothfish from juveniles on the continental shelf to adolescents on the continental slope to adults on the Pacific Antarctic Ridge demonstrates the ontogenetic progression within the Ross Sea following the local bottom water currents in each area. Similar characteristics are observed in the Amundsen Sea and the Bellingshausen Sea but with some clear differences that suggest a high level of mixing of juveniles in the eastern Amundsen Sea and Bellingshausen Sea. The smallest fish (~60 cm) are not observed in the Ross Sea but are observed mainly near 1200 m in both the Amundsen and Bellingshausen Seas. Interestingly, 75–110 cm fish are not frequently observed in the Amundsen or Bellingshausen Seas. With few seamounts in the Bellingshausen Sea, adult fish are well represented on both the continental shelf and slope. Dispersal simulations from locations of adults suggest that Ross Sea juveniles recruit to the Amundsen continental slope, while Amundsen and Bellingshausen Sea juveniles are typically distributed to the continental slope of the Bellingshausen Sea. These patterns of pelagic advection during the first two years in the plankton followed by a slow, down-current ontogenetic migrations returning adults to deep seamount habitats over a 10–15 year period suggesting a metapopulation structure with largely resident regional adult populations with highly variable amounts of juvenile mixing.

This paper updates the research and monitoring activities for New Zealand relevant to the Ross Sea region Marine Protected Area, as encouraged by Conservation Measure 91-05 paragraph 16(i)–(ii).

In 2016 the Commission for the Conservation of Antarctic Marine Living Resources adopted Conservation Measure 91-05, establishing the Ross Sea region Marine Protected Area. Annex B of this Conservation Measure specifies the specific objectives and the Management Plan for this Marine Protected Area. In addition, Annex C specifies the Priority elements for Scientific Research and Monitoring, including research and monitoring priorities and research and monitoring questions that should be addressed. We also recall the objectives of the RSRMPA and the research questions for research and monitoring for the RSRMPA.